PXD030532-2

PXD030532 is an original dataset announced via ProteomeXchange.

Title	Cross-linking of the Endolysosomal System Reveals Potential Flotillin Structures and Cargo
Description	Lysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in lysosomal function. In this context, protein complexes play a decisive role, regulating not only metabolic lysosomal processes but also lysosome biogenesis, transport, and interaction with other organelles. Using cross-linking mass spectrometry, we analyzed lysosomes and early endosomes. Based on the identification of 5,376 cross-links, we investigated protein-protein interactions and structures of lysosome- and endosome-related proteins. In particular, we present evidence for a tetrameric assembly of the lysosomal hydrolase PPT1 and a heterodimeric structure of FLOT1/FLOT2 at lysosomes and early endosomes. For FLOT1-/FLOT2-positive early endosomes, we identified >300 proteins presenting putative cargo, and confirmed several substrates for flotillin-dependent endocytosis, including the latrophilin family of adhesion G protein-coupled receptors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:29.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jasjot Singh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-20 01:48:22	ID requested
1	2022-11-29 02:47:46	announced
⏵ 2	2023-11-14 08:54:29	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Lysosomal interactome, cross-linking MS

Dominic Winter
contact affiliation	Winter Lab Institute for Biochemistry and Molecular Biology (IBMB) Nussallee 11 53115 Bonn, Germanz
contact email	dwin@uni-bonn.de
lab head
Jasjot Singh
contact affiliation	University of Bonn
contact email	jasjotsingh@web.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030532
     1. Label: PRIDE project
     2. Name: Cross-linking of the Endolysosomal System Reveals Potential Flotillin Structures and Cargo