PXD030532 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cross-linking of the Endolysosomal System Reveals Potential Flotillin Structures and Cargo |
Description | Lysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in lysosomal function. In this context, protein complexes play a decisive role, regulating not only metabolic lysosomal processes but also lysosome biogenesis, transport, and interaction with other organelles. Using cross-linking mass spectrometry, we analyzed lysosomes and early endosomes. Based on the identification of 5,376 cross-links, we investigated protein-protein interactions and structures of lysosome- and endosome-related proteins. In particular, we present evidence for a tetrameric assembly of the lysosomal hydrolase PPT1 and a heterodimeric structure of FLOT1/FLOT2 at lysosomes and early endosomes. For FLOT1-/FLOT2-positive early endosomes, we identified >300 proteins presenting putative cargo, and confirmed several substrates for flotillin-dependent endocytosis, including the latrophilin family of adhesion G protein-coupled receptors. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-29 |
AnnouncementXML | Submission_2022-11-29_02:47:46.073.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JasjotSingh |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-20 01:48:22 | ID requested | |
⏵ 1 | 2022-11-29 02:47:46 | announced | |
2 | 2023-11-14 08:54:29 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Lysosomal interactome, cross-linking MS |
Contact List
DominicWinter |
contact affiliation | Winter Lab Institute for Biochemistry and Molecular Biology (IBMB) Nussallee 11 53115 Bonn, Germanz |
contact email | dwin@uni-bonn.de |
lab head | |
JasjotSingh |
contact affiliation | University of Bonn |
contact email | jasjotsingh@web.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD030532 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030532
- Label: PRIDE project
- Name: Cross-linking of the Endolysosomal System Reveals Potential Flotillin Structures and Cargo