PXD030456-1

PXD030456 is an original dataset announced via ProteomeXchange.

Title	Simultaneous characterisation of RNA and protein subcellular dynamics
Description	Localisation by Reduction and Reconstruction (LoRRe) is a new method to map RNA and protein subcellular distribution in membrane and membraneless compartments like stress granules. Importantly, all RNA subcellular locations are interrogated simultaneously, enabling proportional localisation of each RNA. Using LoRRe, we obtain the first global overview of RNA and protein re-localisation during the unfolded protein response (UPR). Despite the global translation shutdown, actin cytoskeleton RNAs maintain ER localisation via eIF3D binding. Codon usage and membrane-localisation are discovered to be important features for targeting RNA to granules, and new proteins regulating this process are identified. Overall, the RNA and protein localisation data provides the most comprehensive picture to date of macromolecule subcellular localisation and re-localisation under stress conditions.
HostingRepository	PRIDE
AnnounceDate	2022-01-25
AnnouncementXML	Submission_2022-01-25_04:09:09.623.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rayner Queiroz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 12:08:19	ID requested
⏵ 1	2022-01-25 04:09:09	announced
2	2022-02-21 08:00:07	announced	2022-02-21: Updated project metadata.

Dataset with its publication pending

submitter keyword: LOPIT, dynamics, subcellular localisation, spatial-proteome, TMT

Kathryn Lilley
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	ksl23@cam.ac.uk
lab head
Rayner Queiroz
contact affiliation	University of Cambridge
contact email	rq214@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD030456

PRIDE project URI

• PRIDE
  1. PXD030456
     1. Label: PRIDE project
     2. Name: Simultaneous characterisation of RNA and protein subcellular dynamics