PXD030456 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Simultaneous characterisation of RNA and protein subcellular dynamics |
Description | Existing methods to analyse RNA localisation are constrained to specific RNAs or subcellular niches, precluding the cell-wide mapping of RNA. We present Localisation of RNA (LoRNA), which maps, at once, RNAs to membranous (nucleus, ER and mitochondria) and membraneless compartments (cytosol, nucleolus and phase-separated granules). Simultaneous interrogation of all RNA locations allows the system-wide quantification of RNA proportional distribution and the comprehensive analysis of RNA subcellular dynamics. Moreover, we have re-engineered the LOPIT (Localisation Of Proteins by Isotope Tagging) method, enabling integration with LoRNA, to jointly map RNA and protein subcellular localisation. Applying this framework, we obtain a global re-localisation map for 31839 transcripts and 5314 proteins during the unfolded protein response, uncovering that ER-localised transcripts are more efficiently recruited to stress granules than cytosolic RNAs, and revealing eIF3d is key to sustain cytoskeletal function. Overall, we provide the most exhaustive map to date of RNA and protein subcellular dynamics. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-21 |
AnnouncementXML | Submission_2022-02-21_08:00:06.766.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rayner Queiroz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-16 12:08:19 | ID requested | |
1 | 2022-01-25 04:09:09 | announced | |
⏵ 2 | 2022-02-21 08:00:07 | announced | 2022-02-21: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: dynamics, subcellular localisation, TMT, spatial-proteome,LOPIT |
Contact List
Kathryn Lilley |
contact affiliation | Department of Biochemistry, University of Cambridge |
contact email | ksl23@cam.ac.uk |
lab head | |
Rayner Queiroz |
contact affiliation | University of Cambridge |
contact email | rq214@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030456
- Label: PRIDE project
- Name: Simultaneous characterisation of RNA and protein subcellular dynamics