PXD029988-2

PXD029988 is an original dataset announced via ProteomeXchange.

Title	Subcellular proteomics of murine presomitic mesoderm
Description	We studied the effects of different stimuli on on the subcellular proteome of primary murine presomitic mesoderm tissue (PSM) in two separate experiments. In the first experiment, tissue was treated ex vivo with either fructose-6-phosphate, fructose-1,6-bisphosphate, or control treatment before subcellular fractionation using a subcellular protein fractionation kit (Thermo Fisher Scientific 78840). In the second experiment, PSMs from transgenic and control mice were extracted and subcellularly fractionated as above. Samples were multiplexed using TMT and analyzed on LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:59.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henrik Hammarén
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 10:28:51	ID requested
1	2022-12-07 02:52:46	announced
⏵ 2	2023-11-14 08:54:00	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: fructose-1, subcellular proteomics, TMT,presomitic mesoderm,6-bisphosphate, fructose-6-phosphate, glycolysis, subcellular fractionation, LC-MS/MS

Martin Beck
contact affiliation	Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
contact email	martin.beck@biophys.mpg.de
lab head
Henrik Hammarén
contact affiliation	Genome Biology Unit and Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact email	henrik.hammaren@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD029988

PRIDE project URI

• PRIDE
  1. PXD029988
     1. Label: PRIDE project
     2. Name: Subcellular proteomics of murine presomitic mesoderm