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PXD029988 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSubcellular proteomics of murine presomitic mesoderm
DescriptionWe studied the effects of different stimuli on on the subcellular proteome of primary murine presomitic mesoderm tissue (PSM) in two separate experiments. In the first experiment, tissue was treated ex vivo with either fructose-6-phosphate, fructose-1,6-bisphosphate, or control treatment before subcellular fractionation using a subcellular protein fractionation kit (Thermo Fisher Scientific 78840). In the second experiment, PSMs from transgenic and control mice were extracted and subcellularly fractionated as above. Samples were multiplexed using TMT and analyzed on LC-MS/MS.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-26 10:28:51ID requested
12022-12-07 02:52:46announced
22023-11-14 08:54:00announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: fructose-1, subcellular proteomics, TMT,presomitic mesoderm,6-bisphosphate, fructose-6-phosphate, glycolysis, subcellular fractionation, LC-MS/MS
Contact List
contact affiliationMax Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
contact emailmartin.beck@biophys.mpg.de
lab head
contact affiliationGenome Biology Unit and Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact emailhenrik.hammaren@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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