PXD029918-2

PXD029918 is an original dataset announced via ProteomeXchange.

Title	Evaluation of volumetric absorptive microsampling and mass spectrometry data-independent acquisition in hemoglobin related clinical markers
Description	Data-independent acquisition (DIA) allows comprehensive proteome coverage, and it has the potential to work as a unified protocol to determine a multitude of proteins in blood. Because of its high specificity, mass spectrometry has a high potential to reduce the interferences presented by other assays in the evaluation of blood markers. Here, we combined DIA with volumetric absorptive microsampling (VAMS) and automated proteomics sample processing as a platform to perform clinical markers determination. We evaluate, as a proof-of-concept, two hemoglobin-related clinical biomarkers: glycated hemoglobin (HbA1c) and hemoglobin variants. HbA1c by DIA showed good correlation with the reference method, but method imprecision did not meet the very low imprecision specification for the clinical evaluation of this biomarker. A strategy for identification of Hb variants was developed based on a customized database combined with a workflow for DIA data extraction and rigorous evaluation of peptides. This approach revealed Hb variants not identified by routinely clinical methods such as Hb Woodville and Hb Okayama.
HostingRepository	PanoramaPublic
AnnounceDate	2022-09-25
AnnouncementXML	Submission_2022-09-25_22:26:29.746.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Valdemir Carvalho
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Hex
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-25 20:11:38	ID requested
1	2022-02-21 14:47:56	announced
⏵ 2	2022-09-25 22:26:30	announced	: Added PubMed Id

Lima DA, Schuch RA, Salgueiro JS, Pint, ã, o MCT, Carvalho VM, Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers. J Proteome Res, 21(8):1816-1828(2022) [pubmed]

submitter keyword: Data-independent acquisition, proteomics, volumetric absorptive microsampling, glycated hemoglobin, hemoglobin variants.

Valdemir Carvalho
contact affiliation	Fleury Group
contact email	melechco@gmail.com
lab head
Valdemir Carvalho
contact affiliation	Fleury Group
contact email	melechco@gmail.com
dataset submitter

Panorama Public dataset URI