PXD029918-1
PXD029918 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluation of volumetric absorptive microsampling and mass spectrometry data-independent acquisition in hemoglobin related clinical markers |
| Description | Data-independent acquisition (DIA) allows comprehensive proteome coverage, and it has the potential to work as a unified protocol to determine a multitude of proteins in blood. Because of its high specificity, mass spectrometry has a high potential to reduce the interferences presented by other assays in the evaluation of blood markers. Here, we combined DIA with volumetric absorptive microsampling (VAMS) and automated proteomics sample processing as a platform to perform clinical markers determination. We evaluate, as a proof-of-concept, two hemoglobin-related clinical biomarkers: glycated hemoglobin (HbA1c) and hemoglobin variants. HbA1c by DIA showed good correlation with the reference method, but method imprecision did not meet the very low imprecision specification for the clinical evaluation of this biomarker. A strategy for identification of Hb variants was developed based on a customized database combined with a workflow for DIA data extraction and rigorous evaluation of peptides. This approach revealed Hb variants not identified by routinely clinical methods such as Hb Woodville and Hb Okayama. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2022-02-21 |
| AnnouncementXML | Submission_2022-02-21_14:47:56.288.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Valdemir Carvalho |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Hex; Carbamidomethyl |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-11-25 20:11:38 | ID requested | |
| ⏵ 1 | 2022-02-21 14:47:56 | announced | |
| 2 | 2022-09-25 22:26:30 | announced | : Added PubMed Id |
Publication List
| no publication |
Keyword List
| submitter keyword: Data-independent acquisition, proteomics, volumetric absorptive microsampling, glycated hemoglobin, hemoglobin variants. |
Contact List
| Valdemir Carvalho | |
|---|---|
| contact affiliation | Fleury Group |
| contact email | melechco@gmail.com |
| lab head | |
| Valdemir Carvalho | |
| contact affiliation | Fleury Group |
| contact email | melechco@gmail.com |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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