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PXD029918 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvaluation of volumetric absorptive microsampling and mass spectrometry data-independent acquisition in hemoglobin related clinical markers
DescriptionData-independent acquisition (DIA) allows comprehensive proteome coverage, and it has the potential to work as a unified protocol to determine a multitude of proteins in blood. Because of its high specificity, mass spectrometry has a high potential to reduce the interferences presented by other assays in the evaluation of blood markers. Here, we combined DIA with volumetric absorptive microsampling (VAMS) and automated proteomics sample processing as a platform to perform clinical markers determination. We evaluate, as a proof-of-concept, two hemoglobin-related clinical biomarkers: glycated hemoglobin (HbA1c) and hemoglobin variants. HbA1c by DIA showed good correlation with the reference method, but method imprecision did not meet the very low imprecision specification for the clinical evaluation of this biomarker. A strategy for identification of Hb variants was developed based on a customized database combined with a workflow for DIA data extraction and rigorous evaluation of peptides. This approach revealed Hb variants not identified by routinely clinical methods such as Hb Woodville and Hb Okayama.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterValdemir Carvalho
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Hex
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-25 20:11:38ID requested
12022-02-21 14:47:56announced
22022-09-25 22:26:30announced: Added PubMed Id
Publication List
Lima DA, Schuch RA, Salgueiro JS, Pint, ã, o MCT, Carvalho VM, Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers. J Proteome Res, 21(8):1816-1828(2022) [pubmed]
Keyword List
submitter keyword: Data-independent acquisition, proteomics, volumetric absorptive microsampling, glycated hemoglobin, hemoglobin variants.
Contact List
Valdemir Carvalho
contact affiliationFleury Group
contact emailmelechco@gmail.com
lab head
Valdemir Carvalho
contact affiliationFleury Group
contact emailmelechco@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI