PXD029254-2

PXD029254 is an original dataset announced via ProteomeXchange.

Title	Gill proteome networks explain salinity tolerance of Oreochromis mossambicus
Description	Acclimations of Oreochromis mossambicus to elevated salinity were conducted with multiple rates of salinity increase and duration of exposure to determine the rate-independent maximum salinity limit and the incipient lethal salinity. A data-independent acquisition (DIA) assay library was created for quantitative analysis of over 3000 gill proteins simultaneously in treatments representative of important key zones in the salinity level/duration landscape. From these DIA data, protein networks that represent complex molecular phenotypes associated with salinity acclimation were generated. Acclimations of Oreochromis mossambicus to elevated salinity were conducted with multiple rates of salinity increase and duration of exposure to determine the rate-independent maximum salinity limit and the incipient lethal salinity. A data-independent acquisition (DIA) assay library was created for quantitative analysis of over 3000 gill proteins simultaneously in treatments representative of important key zones in the salinity level/duration landscape. From these DIA data, protein networks that represent complex molecular phenotypes associated with salinity acclimation were generated. Protein fold change (FC) and organismal level performance indicators of salinity tolerance were then correlated. Gill protein networks impacted at extreme salinity levels both above and below the incipient lethal limit include increased energy metabolism, especially upregulation of electron transport chain complex proteins, and significant downregulation of a previously uncharacterized protein which bears strong amino acid sequence similarity to fucolectin. Proteins networks strongly impacted by crossing into the zone of resistance include cell adhesion and extracellular matrix regulation.
HostingRepository	PanoramaPublic
AnnounceDate	2023-08-01
AnnouncementXML	Submission_2023-08-01_11:02:07.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dietmar Kültz
SpeciesList	scientific name: Oreochromis mossambicus; NCBI TaxID: 8127;
ModificationList	Acetyl; Phospho; Dioxidation; Oxidation; Dehydrated; Delta:H(4)C(2); GG; Dimethyl; Methyl; Carbamidomethyl
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 21:23:04	ID requested
1	2021-10-23 20:15:20	announced
⏵ 2	2023-08-01 11:02:07	announced	: Added PubMed Id

Root L, K, ü, ltz D, Effects of pejus and pessimum zone salinity stress on gill proteome networks and energy homeostasis in Oreochromis mossambicus. Proteomics, 24(1-2):e2300121(2024) [pubmed]

submitter keyword: Gill, Tilapia, Osmoregulation, Salinity, Data-independent acquisition, Ecological proteomics

Dietmar Kültz
contact affiliation	University of California, Davis
contact email	dkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliation	University of California, Davis
contact email	dkueltz@ucdavis.edu
dataset submitter

Panorama Public dataset URI