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PXD029254

PXD029254 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGill proteome networks explain salinity tolerance of Oreochromis mossambicus
DescriptionAcclimations of Oreochromis mossambicus to elevated salinity were conducted with multiple rates of salinity increase and duration of exposure to determine the rate-independent maximum salinity limit and the incipient lethal salinity. A data-independent acquisition (DIA) assay library was created for quantitative analysis of over 3000 gill proteins simultaneously in treatments representative of important key zones in the salinity level/duration landscape. From these DIA data, protein networks that represent complex molecular phenotypes associated with salinity acclimation were generated. Acclimations of Oreochromis mossambicus to elevated salinity were conducted with multiple rates of salinity increase and duration of exposure to determine the rate-independent maximum salinity limit and the incipient lethal salinity. A data-independent acquisition (DIA) assay library was created for quantitative analysis of over 3000 gill proteins simultaneously in treatments representative of important key zones in the salinity level/duration landscape. From these DIA data, protein networks that represent complex molecular phenotypes associated with salinity acclimation were generated. Protein fold change (FC) and organismal level performance indicators of salinity tolerance were then correlated. Gill protein networks impacted at extreme salinity levels both above and below the incipient lethal limit include increased energy metabolism, especially upregulation of electron transport chain complex proteins, and significant downregulation of a previously uncharacterized protein which bears strong amino acid sequence similarity to fucolectin. Proteins networks strongly impacted by crossing into the zone of resistance include cell adhesion and extracellular matrix regulation.
HostingRepositoryPanoramaPublic
AnnounceDate2023-08-01
AnnouncementXMLSubmission_2023-08-01_11:02:07.063.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDietmar Kültz
SpeciesList scientific name: Oreochromis mossambicus; NCBI TaxID: 8127;
ModificationListAcetyl; Phospho; Dioxidation; Oxidation; Dehydrated; Delta:H(4)C(2); GG; Dimethyl; Methyl; Carbamidomethyl
Instrumentimpact II
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-20 21:23:04ID requested
12021-10-23 20:15:20announced
22023-08-01 11:02:07announced: Added PubMed Id
Publication List
Root L, K, ü, ltz D, Effects of pejus and pessimum zone salinity stress on gill proteome networks and energy homeostasis in Oreochromis mossambicus. Proteomics, 24(1-2):e2300121(2024) [pubmed]
Keyword List
submitter keyword: Gill, Tilapia, Osmoregulation, Salinity, Data-independent acquisition, Ecological proteomics
Contact List
Dietmar Kültz
contact affiliationUniversity of California, Davis
contact emaildkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliationUniversity of California, Davis
contact emaildkueltz@ucdavis.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI