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PXD028189-2

PXD028189 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe thiolation modification of tRNA is essential for plant immunity
DescriptionPlants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of tRNA ensures efficient decoding during translation. Here we show that tRNA thiolation is required for plant immunity in Arabidopsis. The Arabidopsis cgb mutant is hyper-susceptible to the pathogen Pseudomonas syringae. CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation. ROL5 physically interacts with CTU2, a homolog of yeast NCS2. Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation. Further analyses reveal that tRNA thiolation is required for both transcriptional reprogramming and translational reprogramming during immune responses. The translation efficiency of immune-related proteins reduces when tRNA thiolation is absent. Our study not only uncovers a new biological function of tRNA thiolation but also reveals a new mechanism for plant immunity.
HostingRepositoryiProX
AnnounceDate2022-07-05
AnnouncementXMLSubmission_2022-07-04_20:23:37.352.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXueao Zheng
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListTMT6plex reporter fragment
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-01 04:53:57ID requested
12021-09-01 04:54:12announced
22022-07-04 20:23:37announced2022-07-05: Update information.
32024-02-29 18:17:10announced2022-07-05: Update information.
2024-03-01: Update information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: tRNA thiolation, translation, plant immunity, Arabidopsis
Contact List
Shunping Yan
contact affiliationHuazhong Agricultural University
contact emailspyan@mail.hzau.edu.cn
lab head
Xueao Zheng
contact affiliationHuazhong Agricultural University
contact emailzhengxueao@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI