PXD028189
PXD028189 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The thiolation modification of tRNA is essential for plant immunity |
| Description | Plants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of tRNA ensures efficient decoding during translation. Here we show that tRNA thiolation is required for plant immunity in Arabidopsis. The Arabidopsis cgb mutant is hyper-susceptible to the pathogen Pseudomonas syringae. CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation. ROL5 physically interacts with CTU2, a homolog of yeast NCS2. Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation. Further analyses reveal that tRNA thiolation is required for both transcriptional reprogramming and translational reprogramming during immune responses. The translation efficiency of immune-related proteins reduces when tRNA thiolation is absent. Our study not only uncovers a new biological function of tRNA thiolation but also reveals a new mechanism for plant immunity. |
| HostingRepository | iProX |
| AnnounceDate | 2022-07-05 |
| AnnouncementXML | Submission_2024-02-29_18:17:09.300.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xueao Zheng |
| SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
| ModificationList | TMT6plex reporter fragment |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-09-01 04:53:57 | ID requested | |
| 1 | 2021-09-01 04:54:12 | announced | |
| 2 | 2022-07-04 20:23:37 | announced | 2022-07-05: Update information. |
| ⏵ 3 | 2024-02-29 18:17:10 | announced | 2022-07-05: Update information. 2024-03-01: Update information. |
Publication List
| Zheng X, Chen H, Deng Z, Wu Y, Zhong L, Wu C, Yu X, Chen Q, Yan S, The tRNA thiolation-mediated translational control is essential for plant immunity. Elife, 13():(2024) [pubmed] |
Keyword List
| submitter keyword: tRNA thiolation, translation, plant immunity, Arabidopsis |
Contact List
| Shunping Yan | |
|---|---|
| contact affiliation | Huazhong Agricultural University |
| contact email | spyan@mail.hzau.edu.cn |
| lab head | |
| Xueao Zheng | |
| contact affiliation | Huazhong Agricultural University |
| contact email | zhengxueao@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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