PXD027588-2

PXD027588 is an original dataset announced via ProteomeXchange.

Title	Deciphering the major metabolic pathways associated with aluminum tolerance in popcorn roots using label-free quantitative proteomics
Description	Aluminum toxicity is one of the most important abiotic stresses that affects crop production worldwide. The soluble form (Al3+) inhibits root growth, altering water and nutrients uptake reducing the plant growth and development. Under a long term of Al3+ exposure, plants can activate several tolerance mechanisms and, to date, there are no reports of large-scale proteomic data of maize in response to this ion. To investigate the post-transcriptional regulation in response to Al toxicity, we performed a label-free quantitative proteomics for comparative analysis of two Al-contrasting popcorn inbred lines and an Al-tolerant commercial hybrid during 72 h under Al-stress. A total of 489 differentially accumulated proteins (DAPs) were identified in the Al-sensitive inbred line, 491 in the Al-tolerant inbred line, and 277 in the commercial hybrid. Among then, 120 DAPs were co-expressed in both Al tolerant genotypes. Bioinformatics analysis indicated that starch and sucrose metabolism, glycolysis/gluconeogenesis, and carbohydrate metabolism were significant biochemical process regulated in response to Al toxicity. The up-accumulation of sucrose synthase and the increased of sucrose content and starch degradation suggest that these components may be enhance popcorn tolerance to Al stress. The up-accumulation of citrate synthase suggests a key role of this enzyme in the detoxification process in the Al-tolerant inbred line. The integration of transcriptomic and proteomic data indicated that Al tolerance response presents a complex regulatory network into the transcription and translation dynamics of popcorn roots development.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:08.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vitor Pinto
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 07:26:47	ID requested
1	2023-05-22 11:03:09	announced
⏵ 2	2023-11-14 08:53:09	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: metabolic pathways, label-free proteomics, popcorn root,Aluminum stress

José Marcelo Soriano Viana
contact affiliation	General Biology Department, Universidade Federal de Viçosa, Brazil
contact email	jmsviana@ufv.br
lab head
Vitor Pinto
contact affiliation	Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)
contact email	vitorbp@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD027588

PRIDE project URI

• PRIDE
  1. PXD027588
     1. Label: PRIDE project
     2. Name: Deciphering the major metabolic pathways associated with aluminum tolerance in popcorn roots using label-free quantitative proteomics