PXD027588-1

PXD027588 is an original dataset announced via ProteomeXchange.

Title	Deciphering the major metabolic pathways associated with aluminum tolerance in popcorn roots using label-free quantitative proteomics
Description	Aluminum toxicity is one of the most important abiotic stresses that affects crop production worldwide. The soluble form (Al3+) inhibits root growth, altering water and nutrients uptake reducing the plant growth and development. Under a long term of Al3+ exposure, plants can activate several tolerance mechanisms and, to date, there are no reports of large-scale proteomic data of maize in response to this ion. To investigate the post-transcriptional regulation in response to Al toxicity, we performed a label-free quantitative proteomics for comparative analysis of two Al-contrasting popcorn inbred lines and an Al-tolerant commercial hybrid during 72 h under Al-stress. A total of 489 differentially accumulated proteins (DAPs) were identified in the Al-sensitive inbred line, 491 in the Al-tolerant inbred line, and 277 in the commercial hybrid. Among then, 120 DAPs were co-expressed in both Al tolerant genotypes. Bioinformatics analysis indicated that starch and sucrose metabolism, glycolysis/gluconeogenesis, and carbohydrate metabolism were significant biochemical process regulated in response to Al toxicity. The up-accumulation of sucrose synthase and the increased of sucrose content and starch degradation suggest that these components may be enhance popcorn tolerance to Al stress. The up-accumulation of citrate synthase suggests a key role of this enzyme in the detoxification process in the Al-tolerant inbred line. The integration of transcriptomic and proteomic data indicated that Al tolerance response presents a complex regulatory network into the transcription and translation dynamics of popcorn roots development.
HostingRepository	PRIDE
AnnounceDate	2023-05-22
AnnouncementXML	Submission_2023-05-22_11:03:08.835.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VitorPinto
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 07:26:47	ID requested
⏵ 1	2023-05-22 11:03:09	announced
2	2023-11-14 08:53:09	announced	2023-11-14: Updated project metadata.

submitter keyword: metabolic pathways, label-free proteomics, popcorn root,Aluminum stress

José Marcelo SorianoViana
contact affiliation	General Biology Department, Universidade Federal de Viçosa, Brazil
contact email	jmsviana@ufv.br
lab head
VitorPinto
contact affiliation	Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)
contact email	vitorbp@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027588
     1. Label: PRIDE project
     2. Name: Deciphering the major metabolic pathways associated with aluminum tolerance in popcorn roots using label-free quantitative proteomics