PXD026741-2

PXD026741 is an original dataset announced via ProteomeXchange.

Title	Proteomic Investigation of Multiple Methicillin-Resistant Staphylococcus aureus strains with distinct Daptomycin Tolerance and Resistance Phenotypes
Description	Several groups have shown that through evolution experiments, tolerance and resistance evolved rapidly under cyclic antibiotic treatment. In other words, intermittent antibiotic exposure performed in a typical adaptive laboratory evolution (ALE) experiments will “train” the bacteria to become tolerant/resistant to the drug. Using this experimental strategy, we performed in vitro laboratory evolution in MRSA using daptomycin, and mine novel daptomycin tolerance and resistance mutants, which were isolated at specific time points during the evolution experiments. Three daptomycin-tolerant isolates with different tolerance level were generated from our laboratory evolution (TOL2 and TOL5 with a mild-tolerance phenotype, and TOL6 with a high-tolerance phenotype). They all bear mutations at different genes, and have no increase in MIC towards daptomycin. Besides, we also isolated three daptomycin-resistant isolates (RES1, RES2, RES3) that have a single point mutation in the same gene, mprF, but at different locations, leading to an increased MIC towards daptomycin. Through proteomics, we uncovered the differential adaptation strategies of these daptomycin tolerant and resistant MRSA strains, and how they respond differently to antibiotics compared to the ancestral wild-type.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:11.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordy Evan Sulaiman
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-16 04:11:52	ID requested
1	2022-07-13 22:40:08	announced
⏵ 2	2023-11-14 08:52:12	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, daptomycin,Methicillin-resistant Staphylococcus aureus, adaptive laboratory evolution, tolerance

Henry Lam
contact affiliation	Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST)
contact email	kehlam@ust.hk
lab head
Jordy Evan Sulaiman
contact affiliation	The Hong Kong University of Science and Technology (HKUST)
contact email	jesulaiman@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD026741

PRIDE project URI

• PRIDE
  1. PXD026741
     1. Label: PRIDE project
     2. Name: Proteomic Investigation of Multiple Methicillin-Resistant Staphylococcus aureus strains with distinct Daptomycin Tolerance and Resistance Phenotypes