PXD026741 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Investigation of Multiple Methicillin-Resistant Staphylococcus aureus strains with distinct Daptomycin Tolerance and Resistance Phenotypes |
| Description | Several groups have shown that through evolution experiments, tolerance and resistance evolved rapidly under cyclic antibiotic treatment. In other words, intermittent antibiotic exposure performed in a typical adaptive laboratory evolution (ALE) experiments will “train” the bacteria to become tolerant/resistant to the drug. Using this experimental strategy, we performed in vitro laboratory evolution in MRSA using daptomycin, and mine novel daptomycin tolerance and resistance mutants, which were isolated at specific time points during the evolution experiments. Three daptomycin-tolerant isolates with different tolerance level were generated from our laboratory evolution (TOL2 and TOL5 with a mild-tolerance phenotype, and TOL6 with a high-tolerance phenotype). They all bear mutations at different genes, and have no increase in MIC towards daptomycin. Besides, we also isolated three daptomycin-resistant isolates (RES1, RES2, RES3) that have a single point mutation in the same gene, mprF, but at different locations, leading to an increased MIC towards daptomycin. Through proteomics, we uncovered the differential adaptation strategies of these daptomycin tolerant and resistant MRSA strains, and how they respond differently to antibiotics compared to the ancestral wild-type. |
| HostingRepository | PRIDE |
| AnnounceDate | 2022-07-14 |
| AnnouncementXML | Submission_2022-07-13_22:40:08.526.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jordy Evan Sulaiman |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2021-06-16 04:11:52 | ID requested | |
| ⏵ 1 | 2022-07-13 22:40:08 | announced | |
| 2 | 2023-11-14 08:52:12 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: Methicillin-resistant Staphylococcus aureus, daptomycin, tolerance, adaptive laboratory evolution, proteomics |
Contact List
| Henry Lam |
|---|
| contact affiliation | Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST) |
| contact email | kehlam@ust.hk |
| lab head | |
| Jordy Evan Sulaiman |
|---|
| contact affiliation | The Hong Kong University of Science and Technology (HKUST) |
| contact email | jesulaiman@connect.ust.hk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026741
- Label: PRIDE project
- Name: Proteomic Investigation of Multiple Methicillin-Resistant Staphylococcus aureus strains with distinct Daptomycin Tolerance and Resistance Phenotypes
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