PXD026665-1
PXD026665 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Modeling lignin biosynthesis from proteomics and isotopic labeling data in Brachypodium distachyon |
| Description | Previous studies suggest the existence of parallel biosynthetic routes to the building blocks of lignin. Here, using the model grass Brachypodium distachyon, we investigated these routes through a combined proteomics and isotope labeling approach. The key enzymes involved in the formation of phenolic acids L-phenylalanine/tyrosine ammonia-lyase (PTAL), p-coumarate 3-hydroxylase (C3H) and caffeic acid 3-O-methyltransferase (COMT) were among the most abundant of the 11,417 proteins identified in mature stems. A set of double RNAi-knockdown lines targeting putative parallel monolignol pathways did not produce additive effects on lignin deposition or growth defects, but instead induced pronounced proteome changes associated with oxidative stress and vitamin E synthesis. 13C-Labeling data, metabolic flux analysis and in situ hybridization experiments supported distinct but partially overlapping routes from L-phenylalanine and L-tyrosine into different lignin subunits and flavonoid classes associated with distinct cellular localization. These results provide a deeper understanding of the lignification process in grasses. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-06-02 |
| AnnouncementXML | Submission_2022-06-02_07:55:44.500.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | CBI_lignin_project |
| SpeciesList | scientific name: Brachypodium distachyon; common name: stiff brome; NCBI TaxID: 15368; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-06-11 08:57:32 | ID requested | |
| ⏵ 1 | 2022-06-02 07:55:44 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Brachypodium distachyon, lignin, proteomics, isotopic labeling |
Contact List
| Paul E. Abraham | |
|---|---|
| contact affiliation | Oak Ridge National Laboratory |
| contact email | abrahampe@ornl.gov |
| lab head | |
| CBI_lignin_project | |
| contact affiliation | ORNL |
| contact email | shresthahk@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087614/ |
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