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<DatasetSummary announceDate="2022-06-02" hostingRepository="MassIVE" title="Modeling lignin biosynthesis from proteomics and isotopic labeling data in Brachypodium distachyon">
<Description><![CDATA[Previous studies suggest the existence of parallel biosynthetic routes to the building blocks of lignin. Here, using the model grass Brachypodium distachyon, we investigated these routes through a combined proteomics and isotope labeling approach. The key enzymes involved in the formation of phenolic acids L-phenylalanine/tyrosine ammonia-lyase (PTAL), p-coumarate 3-hydroxylase (C3H) and caffeic acid 3-O-methyltransferase (COMT) were among the most abundant of the 11,417 proteins identified in mature stems. A set of double RNAi-knockdown lines targeting putative parallel monolignol pathways did not produce additive effects on lignin deposition or growth defects, but instead induced pronounced proteome changes associated with oxidative stress and vitamin E synthesis. 13C-Labeling data, metabolic flux analysis and in situ hybridization experiments supported distinct but partially overlapping routes from L-phenylalanine and L-tyrosine into different lignin subunits and flavonoid classes associated with distinct cellular localization. These results provide a deeper understanding of the lignification process in grasses.]]></Description>
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<cvParam accession="MS:1000589" cvRef="MS" name="contact email" value="shresthahk@ornl.gov"/>
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<cvParam accession="MS:1000586" cvRef="MS" name="contact name" value="Paul E. Abraham"/>
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