PXD026622-1

PXD026622 is an original dataset announced via ProteomeXchange.

Title	The RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei
Description	RNA-binding proteins are key players in coordinated post-transcriptional regulation of functionally related genes, defined as RNA regulons. RNA regulons play particularly critical roles in parasitic trypanosomes, which exhibit unregulated co-transcription of long unrelated gene arrays. In this report, we present a systematic analysis of an essential RNA-binding protein, RBP42, in the mammalian-infective bloodstream form of African trypanosome, and show that RBP42 is a key regulator of parasite’s central carbon and energy metabolism. Using individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to identify genome-wide RBP42-RNA interactions, we show that RBP42 preferentially binds within the coding region of mRNAs encoding core metabolic enzymes. Global quantitative transcriptomic and proteomic analyses reveal that loss of RBP42 reduces the abundance of target mRNA-encoded proteins, but not target mRNA, suggesting a positive translational regulatory role of RBP42. Significant changes in central carbon metabolic intermediates, following loss of RBP42, further support its critical role in cellular energy metabolism.
HostingRepository	PRIDE
AnnounceDate	2021-06-11
AnnouncementXML	Submission_2021-06-10_22:23:55.000.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026622
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tong Liu
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 09:03:55	ID requested
⏵ 1	2021-06-10 22:23:55	announced

Dataset with its publication pending

submitter keyword: RBP42, Trypanosoma brucei, LC-MS/MS, iTRAQ

Anish Das
contact affiliation	Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers-New Jersey Medical School, Newark, NJ 07103
contact email	dasak@njms.rutgers.edu
lab head
Tong Liu
contact affiliation	Rutgers University
contact email	linto@njms.rutgers.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026622
     1. Label: PRIDE project
     2. Name: The RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei