PXD026564-3

PXD026564 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic and mutant analysis of hydrogenase gene hypE in symbiotic nitrogen fixation in Mesorhizobium huakuii
Description	Hydrogenases catalyze the simple yet importantredox reaction between protons and electrons and H2, thus, mediate interactions between microorganisms and their chemical environment. A mutation in M. huakuii hypE gene was generated by homologous recombination. The hypE mutant grew a little faster than its parental 7653R. Real-time quantitative PCR showed that the hypE gene expression is significantly upregulated in all the detected stages of nodule development. The wild type contained normal spherical shape of the nodules, while the hypE mutant elicited small-size nodules. Plants inoculated with mutant HKhypE showed a significantly more than 47% decrease in acetylene reduction activity compared to the wild-type 7653R. Proteomics analysis showed seven proteins were up-regulated and eighty-two proteins were down-regulated in hypE triple mutant bacteroids.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:07:23.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guojun Cheng
SpeciesList	scientific name: Mesorhizobium huakuii; NCBI TaxID: 28104;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-08 02:15:16	ID requested
1	2023-10-24 07:09:19	announced
2	2023-11-14 09:06:19	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:07:24	announced	2024-10-22: Updated project metadata.

Publication List

Long S, Su M, Chen X, Hu A, Yu F, Zou Q, Cheng G, . Int J Mol Sci, 24(16):(2023) [pubmed]
10.3390/ijms241612534;

Long S, Su M, Chen X, Hu A, Yu F, Zou Q, Cheng G, . Int J Mol Sci, 24(16):(2023) [pubmed]

10.3390/ijms241612534;

Keyword List

submitter keyword: Mesorhizobium huakuii
FmoA
Antioxidant
Symbiotic nitrogen fixation
Proteome & Mesorhizobium huakuii
Hydrogenases
hypE gene
Symbiotic nitrogen fixation
quantitative proteomics

submitter keyword: Mesorhizobium huakuii

FmoA

Antioxidant

Symbiotic nitrogen fixation

Proteome & Mesorhizobium huakuii

Hydrogenases

hypE gene

Symbiotic nitrogen fixation

quantitative proteomics

Contact List

Guojun Cheng
contact affiliation	College of Life Sciences, South-Central University for Nationalities
contact email	chengguojun@mail.scuec.edu.cn
lab head
Guojun Cheng
contact affiliation	College of Life Sciences, South-Central University for Nationalities
contact email	chengguojun@mail.scuec.edu.cn
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD026564

PRIDE project URI

Repository Record List

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