PXD026564 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic and mutant analysis of hydrogenase gene hypE in symbiotic nitrogen fixation in Mesorhizobium huakuii |
| Description | Hydrogenases catalyze the simple yet importantredox reaction between protons and electrons and H2, thus, mediate interactions between microorganisms and their chemical environment. A mutation in M. huakuii hypE gene was generated by homologous recombination. The hypE mutant grew a little faster than its parental 7653R. Real-time quantitative PCR showed that the hypE gene expression is significantly upregulated in all the detected stages of nodule development. The wild type contained normal spherical shape of the nodules, while the hypE mutant elicited small-size nodules. Plants inoculated with mutant HKhypE showed a significantly more than 47% decrease in acetylene reduction activity compared to the wild-type 7653R. Proteomics analysis showed seven proteins were up-regulated and eighty-two proteins were down-regulated in hypE triple mutant bacteroids. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-10-24 |
| AnnouncementXML | Submission_2023-10-24_07:09:18.760.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | GuojunCheng |
| SpeciesList | scientific name: Mesorhizobium huakuii; NCBI TaxID: 28104; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2021-06-08 02:15:16 | ID requested | |
| ⏵ 1 | 2023-10-24 07:09:19 | announced | |
| 2 | 2023-11-14 09:06:19 | announced | 2023-11-14: Updated project metadata. |
| 3 | 2024-10-22 06:07:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Long S, Su M, Chen X, Hu A, Yu F, Zou Q, Cheng G, . Int J Mol Sci, 24(16):(2023) [pubmed] |
Keyword List
| submitter keyword: Mesorhizobium huakuii |
| FmoA |
| Antioxidant |
| Symbiotic nitrogen fixation |
| Proteome & Mesorhizobium huakuii |
| Hydrogenases |
| hypE gene |
| Symbiotic nitrogen fixation |
| quantitative proteomics |
Contact List
| GuojunCheng |
|---|
| contact affiliation | College of Life Sciences, South-Central University for Nationalities |
| contact email | chengguojun@mail.scuec.edu.cn |
| lab head | |
| GuojunCheng |
|---|
| contact affiliation | College of Life Sciences, South-Central University for Nationalities |
| contact email | chengguojun@mail.scuec.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026564
- Label: PRIDE project
- Name: Proteomic and mutant analysis of hydrogenase gene hypE in symbiotic nitrogen fixation in Mesorhizobium huakuii
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