PXD026517-2
PXD026517 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Pupylation sites on protein Sox2 |
| Description | As a test for the effectiveness of SPIDER with RNA-protein pairs, We used Sox2 and specific biotin-DNA and biotin-RNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on Sox2 after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e., GGE, that covalently binds to adjacent lysine by pupylation. ） |
| HostingRepository | iProX |
| AnnounceDate | 2021-06-04 |
| AnnouncementXML | Submission_2023-05-06_00:12:47.032.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xuening Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-06-06 22:21:00 | ID requested | |
| 1 | 2021-06-06 22:21:23 | announced | |
| ⏵ 2 | 2023-05-06 00:12:47 | announced | 2023-05-06: Update publication information. |
Publication List
| Jiang HW, Chen H, Zheng YX, Wang XN, Meng Q, Xie J, Zhang J, Zhang C, Xu ZW, Chen ZQ, Wang L, Kong WS, Zhou K, Ma ML, Zhang HN, Guo SJ, Xue JB, Hou JL, Liu ZY, Niu WX, Wang FJ, Wang T, Li W, Wang RN, Dang YJ, Czajkowsky DM, Pei J, Dong JJ, Tao SC, Specific pupylation as IDEntity reporter (SPIDER) for the identification of protein-biomolecule interactions. Sci China Life Sci, 66(8):1869-1887(2023) [pubmed] |
Keyword List
| submitter keyword: Homo sapiens, Sox2, pupylation |
Contact List
| Shengce Tao | |
|---|---|
| contact affiliation | Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong Universit |
| contact email | taosc@sjtu.edu.cn |
| lab head | |
| Xuening Wang | |
| contact affiliation | Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong Universit |
| contact email | wxnyoyo@sjtu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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