PXD024636 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Inhibition of cathepsin B and SAPC secreted by HIV-infected macrophages reverse common and unique apoptosis pathways |
Description | Human immunodeficiency virus 1 (HIV-1) infects blood monocytes that cross the blood-brain barrier to the central nervous system inducing neuronal damage. This damage is prompted by the secretion of viral and neurotoxic factors by HIV-infected macrophages and can result in HIV associated neurocognitive disorders (HAND). One of these neurotoxic factors secreted by HIV-infected macrophages is cathepsin B (CATB), a lysosomal cysteine protease that plays an important role in neurodegeneration. CATB interacts with Serum Amyloid P component (SAPC) contributing to HIV-induced neurotoxicity. However, the neuronal apoptosis pathways triggered by CATB and SAPC remain unknown. We aimed to elucidate these pathways in neurons exposed to HIV-infected macrophage conditioned media (MCM) before and after inhibition of CATB or SAPC using Tandem Mass Tag (TMT) proteomics labeling. Based on significant fold change (FC) ≥ ǀ2ǀ and p-value < 0.05 criteria, a total of 10, 48 and 13 proteins were deregulated after inhibiting CATB, SAPC antibodies and the cathepsin B inhibitor CA-074, respectively. We found that antibodies against CATB and SAPC, as well as the CATB inhibitor CA-074 downregulated apoptosis related proteins. CATB, SAPC or apoptotic related proteins could become potential targets against HIV-induced neuronal degeneration. |
HostingRepository | PRIDE |
AnnounceDate | 2022-01-20 |
AnnouncementXML | Submission_2022-01-20_10:03:38.502.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD024636 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yadira Cantres |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-10 02:22:45 | ID requested | |
1 | 2022-01-20 10:00:59 | announced | |
⏵ 2 | 2022-01-20 10:03:39 | announced | 2022-01-20: Updated publication reference for PubMed record(s): 34994563. |
Publication List
Zen, ó, n-Mel, é, ndez CN, Carrasquillo Carri, ó, n K, Cantres Rosario Y, Roche Lima A, Mel, é, ndez LM, Inhibition of Cathepsin B and SAPC Secreted by HIV-Infected Macrophages Reverses Common and Unique Apoptosis Pathways. J Proteome Res, 21(2):301-312(2022) [pubmed] |
Keyword List
submitter keyword: HIV-1, Cathepsin B, SAPC, HAND, Apoptosis, TMT |
Contact List
Loyda Melendez |
contact affiliation | University of Puerto Rico, Medical Sciences Campus, Microbioly and Zoology Department, Translational Proteomics Center |
contact email | loyda.melendez@upr.edu |
lab head | |
Yadira Cantres |
contact affiliation | Universtity of Puerto Rico, Comprehensive Cancer Center |
contact email | yadira.cantres@upr.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024636
- Label: PRIDE project
- Name: Inhibition of cathepsin B and SAPC secreted by HIV-infected macrophages reverse common and unique apoptosis pathways