PXD024428-2

PXD024428 is an original dataset announced via ProteomeXchange.

Title	Stage-specific, morphological and molecular markers of encystation in Giardia lamblia
Description	Differentiation into environmentally resistant cysts is required for transmission of the ubiquitous intestinal parasite Giardia lamblia. Encystation in Giardia requires the production, processing and transport of Cyst Wall Proteins (CWPs) in stage induced Golgi-like Encystation Specific Vesicles (ESVs). While progress of this trafficking pathway can be followed by tracking CWPs, there is no recognized system to distinguish stages of this process that takes varied lengths of time to complete depending on the encystation method used. Here we propose a staging system for encysting Giardia based on the morphology of CWP1-stained ESVs. The molecular distinctiveness of ESVs at these stages were demonstrated by following Giardia Rabs through encystation. We previously demonstrated that Giardia’s sole Rho family GTPase, GlRac, associates with ESVs and has a role in regulating CWP1 maturation and secretion. As a proof of principle, we delineated the relationship among GlRac and ESV stages and through proteomic studies identified putative interactors of GlRac that could be used as additional ESV Stage-specific markers. We propose that this staging system provides a common descriptor of ESV maturation regardless of the source of encysting cells and the identified set of molecular markers for different ESV stages will be a powerful tool for characterizing trafficking mutants that impair typical ESV maturation and morphology.
HostingRepository	PRIDE
AnnounceDate	2021-07-12
AnnouncementXML	Submission_2021-07-11_22:11:37.672.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingyun Zheng
SpeciesList	scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 04:44:25	ID requested
1	2021-07-11 04:06:19	announced
⏵ 2	2021-07-11 22:11:38	announced	2021-07-12: Updated project metadata.

Thomas EB, Sutanto R, Johnson RS, Shih HW, Alas GCM, Krtkov, á J, MacCoss MJ, Paredez AR, Using Encystation-Specific Vesicle Morphology and Associating Molecular Markers. Front Cell Dev Biol, 9():662945(2021) [pubmed]

10.1038/S41589-021-00823-5;

submitter keyword: Rho GTPase,Giardia, Membrane trafficking, Rac, Encystation

Lie Liu
contact affiliation	Tsinghua university, Department of Chemistry
contact email	lliu@tsinghua.edu.cn
lab head
Qingyun Zheng
contact affiliation	Department of Chemistry, Tsinghua University
contact email	zhengqytsinghua@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024428

PRIDE project URI

• PRIDE
  1. PXD024428
     1. Label: PRIDE project
     2. Name: Stage-specific, morphological and molecular markers of encystation in Giardia lamblia