PXD024428-1

PXD024428 is an original dataset announced via ProteomeXchange.

Title	To identify proteins related with K29-linked ubiquitination using sAB-K29
Description	Protein ubiquitination shows remarkable topological and functional diversity through the polymerization of ubiquitin via different linkages. Among the eight possible linkages, K29-linked polyubiquitin is a relatively abundant type of polyubiquitin in both human and yeast cells. However, our understanding of its function is rather limited due to the lack of specific binders as tools to detect K29-linked polyubiquitin. We screened and characterized a synthetic antigen-binding fragment, termed sAB-K29, that can specifically recognize K29-linked polyubiquitin. Using sAB-K29 as a tool, we uncovered that K29-linked ubiquitination is involved in different kinds of cellular proteotoxic stress response as well as cell cycle regulation.
HostingRepository	PRIDE
AnnounceDate	2021-07-11
AnnouncementXML	Submission_2021-07-11_04:06:19.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingyun Zheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 04:44:25	ID requested
⏵ 1	2021-07-11 04:06:19	announced
2	2021-07-11 22:11:38	announced	2021-07-12: Updated project metadata.

10.1038/S41589-021-00823-5;

submitter keyword: K29-linked ubiquitination

Lie Liu
contact affiliation	Tsinghua university, Department of Chemistry
contact email	lliu@tsinghua.edu.cn
lab head
Qingyun Zheng
contact affiliation	Department of Chemistry, Tsinghua University
contact email	zhengqytsinghua@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024428

PRIDE project URI

• PRIDE
  1. PXD024428
     1. Label: PRIDE project
     2. Name: To identify proteins related with K29-linked ubiquitination using sAB-K29