PXD024096-2

PXD024096 is an original dataset announced via ProteomeXchange.

Title	Investigation of the host response of naïve Atlantic salmon gill to repeat inoculation with Paramoeba perurans
Description	In aquaculture, recurrence rates of Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans (P. perurans) are high and no prophylactic strategies exist for disease prevention. In this study, Atlantic salmon (Salmo salar) were initially inoculated with P. perurans and following the development of amoebic gill disease were treated with fresh water immersion on day 21 and day 35 post inoculation. Fish were re-inoculated following a negative Q-PCR analysis for the presence of P. perurans. The gill host immune response was investigated at 7, 14 and 18 days post re-inoculation. Differential proteome expression of immune related proteins was assessed by comparison of each time point against naïve controls. In the gill, some proteins of the innate immune system were expressed in response to gill re-colonisation by P. perurans, while no features of adaptive immunity were found to be differentially expressed. Many of the proteins identified are novel in the context of AGD and their expression profiles suggest that their roles in the response to disease development and progression in single or multiple infections warrant further investigation.
HostingRepository	PRIDE
AnnounceDate	2021-05-12
AnnouncementXML	Submission_2021-05-12_04:12:16.450.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eugene Dillon
SpeciesList	scientific name: Salmo salar (Atlantic salmon); NCBI TaxID: 8030;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-09 10:40:23	ID requested
1	2021-05-12 01:00:15	announced
⏵ 2	2021-05-12 04:12:17	announced	2021-05-12: Updated project metadata.

McCormack M, Dillon E, O'Connor I, MacCarthy E, . Microorganisms, 9(4):(2021) [pubmed]

10.3390/MICROORGANISMS9040746;

submitter keyword: gill, proteomics,salmon

Michelle McCormack
contact affiliation	Marine and Freshwater Research Centre, Galway Mayo Institute of Technology, Dublin Road, Galway, Ireland, H91 TRNW
contact email	michelle.mccormack@gmit.ie
lab head
Eugene Dillon
contact affiliation	UCD
contact email	eugene.dillon@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024096

PRIDE project URI

• PRIDE
  1. PXD024096
     1. Label: PRIDE project
     2. Name: Investigation of the host response of naïve Atlantic salmon gill to repeat inoculation with Paramoeba perurans