PXD023510 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2 |
Description | Ribosome biogenesis is an essential process within cells that requires integration of extracellular cues such as metabolic states through signaling with transcriptional regulation and maintenance of accessible chromatin at the ribosomal DNA. Here, we demonstrate that the recently identified histone modification, methylation of H2AQ105 is an integral part of a dynamic chromatin network at the rDNA locus. Its deposition depends on a functional mTor signaling pathway and acetylation of histone H3 at position K56, thus integrating signals from cell cycle and proliferative states. Furthermore, we identify a first epigenetic reader of this modification. The ribonucleoprotein Nhp2 specifically recognizes methylation H2AQ105me. Based on functional and proteomic data we suggest that Nhp2 functions as an adapter to bridge chromatin and components of the small subunit processome and might help to efficiently coordinate transcription of rRNA with its post-transcriptional processing. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-06 |
AnnouncementXML | Submission_2021-08-05_22:01:52.030.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ilian Atanassov |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-11 07:15:44 | ID requested | |
⏵ 1 | 2021-08-05 22:01:52 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SILAC, LFQ, IP |
Contact List
Peter Tessarz |
contact affiliation | Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, 50931 Cologne, Germany Cologne Excellence Cluster on Stress Responses in ageing-associated Diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50931 15Cologne, Germany |
contact email | ptessarz@age.mpg.de |
lab head | |
Ilian Atanassov |
contact affiliation | Max Planck Institute for Biology of Aging |
contact email | Ilian.Atanassov@age.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD023510
- Label: PRIDE project
- Name: Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2