PXD022121-2
PXD022121 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Tyrosine Reactive Cross-Linker for Probing Protein 3D Structures |
Description | Cross-linking mass spectrometry (XL-MS) has made significant progress in understanding the structure of protein and elucidating architectures of larger protein complexes. Current XL-MS applications are limited to targeting lysine, glutamic acid, aspartic acid, and cysteine residues. There remains a need for the development of novel cross-linkers enabling selectively target other amino-acid residues in proteins. Here, a novel simple cross-linker, namely [4,4'-(disulfanediylbis(ethane-2,1-diyl)) bis(1,2,4-triazolidine-3,5-dione)] (DBB), has been designed, synthesized, and characterized. This cross-linker can react selectively with tyrosine residues in protein through electrochemical click reaction. Upon tandem mass spectrometry, the DBB cross-links produced the characteristic fragments, thus permitting the simplified data analysis and accurate identification for the cross-linked products. This is the first time developing a cross-linker for targeting tyrosine residue on protein without using photoirradiation or metal catalyst. This strategy might potentially be used as a complementary tool for XL-MS to probe protein 3D structures, protein complexes, and protein-protein interaction. |
HostingRepository | iProX |
AnnounceDate | 2020-10-22 |
AnnouncementXML | Submission_2023-08-24_21:06:37.763.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lili Cui |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker BAF format |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-10-21 20:25:31 | ID requested | |
1 | 2020-10-21 20:31:50 | announced | |
⏵ 2 | 2023-08-24 21:06:38 | announced | 2023-08-25: Update publication information. |
Publication List
Cui L, Ma Y, Li M, Wei Z, Huan Y, Li H, Fei Q, Zheng L, Tyrosine-Reactive Cross-Linker for Probing Protein Three-Dimensional Structures. Anal Chem, 93(10):4434-4440(2021) [pubmed] |
Keyword List
submitter keyword: Tyrosine, Cross-linking mass spectrometry |
Contact List
Ming Li | |
---|---|
contact affiliation | National Institute of Metrology |
contact email | mingutah@hotmail.com |
lab head | |
Lili Cui | |
contact affiliation | Jilin University |
contact email | 18317724263@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |