PXD022121-1

PXD022121 is an original dataset announced via ProteomeXchange.

Title	A Tyrosine Reactive Cross-Linker for Probing Protein 3D Structures
Description	Cross-linking mass spectrometry (XL-MS) has made significant progress in understanding the structure of protein and elucidating architectures of larger protein complexes. Current XL-MS applications are limited to targeting lysine, glutamic acid, aspartic acid, and cysteine residues. There remains a need for the development of novel cross-linkers enabling selectively target other amino-acid residues in proteins. Here, a novel simple cross-linker, namely [4,4'-(disulfanediylbis(ethane-2,1-diyl)) bis(1,2,4-triazolidine-3,5-dione)] (DBB), has been designed, synthesized, and characterized. This cross-linker can react selectively with tyrosine residues in protein through electrochemical click reaction. Upon tandem mass spectrometry, the DBB cross-links produced the characteristic fragments, thus permitting the simplified data analysis and accurate identification for the cross-linked products. This is the first time developing a cross-linker for targeting tyrosine residue on protein without using photoirradiation or metal catalyst. This strategy might potentially be used as a complementary tool for XL-MS to probe protein 3D structures, protein complexes, and protein-protein interaction.
HostingRepository	iProX
AnnounceDate	2020-10-22
AnnouncementXML	Submission_2020-10-21_20:31:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lili Cui
SpeciesList	scientific name: Beaverium insulindicus; NCBI TaxID: 995723;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker BAF format

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-21 20:25:31	ID requested
⏵ 1	2020-10-21 20:31:50	announced
2	2023-08-24 21:06:38	announced	2023-08-25: Update publication information.

Dataset with its publication pending

submitter keyword: Tyrosine , Cross-linking mass spectrometry

Ming Li
contact affiliation	National Institute of Metrology
contact email	mingutah@hotmail.com
lab head
Lili Cui
contact affiliation	Jilin University
contact email	18317724263@163.com
dataset submitter

iProX dataset URI