PXD022028-1

PXD022028 is an original dataset announced via ProteomeXchange.

Title	Host cholesterol modulates the generation and enrichment of persister population during Mycobacterium tuberculosis infection.
Description	BCG strain overexpressing the toxin (vapC12)–antitoxin (vapB12)-His(6X) locus was used for sample preparation. Log phase culture of the overexpressed strain was washed with PBS and inoculated in minimal media with 0.1% glycerol and minimal media with 0.01% cholesterol. The cultures were allowed to grow for 48 hrs and cell lysate was prepared. Immunoprecipitation was performed using an anti-His antibody (BTL1010, Biospecs). In-solution digestion was carried out for 10 µg of proteins from each condition. The samples were subjected to reduction and alkylation using 5 mM dithiothreitol (DTT) (60°C for 45 minutes) and alkylation using 10 mM Iodoacetamide (IAA). Trypsin (Gold mass-spectrometry trypsin; Promega, Madison, WI) digestion was carried out at 37°C for 10-12 hrs. The peptides were vacuum-dried and stored at -80°C until LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2020-10-16
AnnouncementXML	Submission_2020-10-16_06:36:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renu Goel
SpeciesList	scientific name: Mycobacterium tuberculosis M24; NCBI TaxID: 1268063;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-16 03:46:13	ID requested
⏵ 1	2020-10-16 06:36:54	announced
2	2020-11-02 05:33:41	announced	2020-11-02: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, LC-MS/MS

Dr Amit Pandey
contact affiliation	Translational Health Science and Technology Institute
contact email	amitpandey@thsti.res.in
lab head
Renu Goel
contact affiliation	Translational Health Science and technology Institute
contact email	drrenugoel@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD022028

PRIDE project URI

• PRIDE
  1. PXD022028
     1. Label: PRIDE project
     2. Name: Host cholesterol modulates the generation and enrichment of persister population during Mycobacterium tuberculosis infection.