PXD021949-1

PXD021949 is an original dataset announced via ProteomeXchange.

Title	Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP
Description	We use ZooMS to obtain secure species identifications of key specimens of early domesticated fauna from South Africa, dating to ca. 2000 BP. Because it can be difficult to distinguish between fragmentary remains of early domesticates (sheep) and similar-sized local wild bovids (grey duiker, grey rhebok, springbok) based on morphology alone, we explore the use of biomolecular methods to make these distinctions. As well as the traditional method of analysing bone fragments, we show the utility of minimally destructive sampling methods such as PVC eraser and polishing films for successful ZooMS identification. We also show that collagen extracted more than 25 years ago for the purpose of radiocarbon dating can yield successful ZooMS identification. Our study demonstrates the importance of developing appropriate regional frameworks of comparison for future research using ZooMS as a method of biomolecular species identification on archaeological faunal assemblages. We confirm that the specimen from the site of Spoegrivier dated to 2105±65 BP is indeed a sheep. This is the earliest directly dated evidence of domesticated animals in southern Africa.
HostingRepository	PRIDE
AnnounceDate	2021-03-23
AnnouncementXML	Submission_2021-03-23_03:57:08.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meaghan Mackie
SpeciesList	scientific name: Sylvicapra grimmia; NCBI TaxID: 119562; scientific name: Antidorcas marsupialis; NCBI TaxID: 59523; scientific name: Pelea capreolus; NCBI TaxID: 59552; scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	monohydroxylated proline; iodoacetamide derivatized residue; deamidated residue
Instrument	ultraflex; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-13 03:23:06	ID requested
⏵ 1	2021-03-23 03:57:08	announced

Dataset with its publication pending

submitter keyword: collagen peptide mass fingerprinting, ZooMS, LC-MS/MS, animal husbandry, southern African archaeology

Matthew Collins
contact affiliation	Section for Evolutionary Genomics, GLOBE Institute, University of Copenhagen, Copenhagen, Denmark; McDonald Institute for Archaeological Research, University of Cambridge, Cambridge, UK
contact email	matthew.collins@sund.ku.dk
lab head
Meaghan Mackie
contact affiliation	University of Copenhagen
contact email	meaghan@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD021949

PRIDE project URI

• PRIDE
  1. PXD021949
     1. Label: PRIDE project
     2. Name: Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP