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PXD021949-1

PXD021949 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePalaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP
DescriptionWe use ZooMS to obtain secure species identifications of key specimens of early domesticated fauna from South Africa, dating to ca. 2000 BP. Because it can be difficult to distinguish between fragmentary remains of early domesticates (sheep) and similar-sized local wild bovids (grey duiker, grey rhebok, springbok) based on morphology alone, we explore the use of biomolecular methods to make these distinctions. As well as the traditional method of analysing bone fragments, we show the utility of minimally destructive sampling methods such as PVC eraser and polishing films for successful ZooMS identification. We also show that collagen extracted more than 25 years ago for the purpose of radiocarbon dating can yield successful ZooMS identification. Our study demonstrates the importance of developing appropriate regional frameworks of comparison for future research using ZooMS as a method of biomolecular species identification on archaeological faunal assemblages. We confirm that the specimen from the site of Spoegrivier dated to 2105±65 BP is indeed a sheep. This is the earliest directly dated evidence of domesticated animals in southern Africa.
HostingRepositoryPRIDE
AnnounceDate2021-03-23
AnnouncementXMLSubmission_2021-03-23_03:57:08.207.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeaghan Mackie
SpeciesList scientific name: Sylvicapra grimmia; NCBI TaxID: 119562; scientific name: Antidorcas marsupialis; NCBI TaxID: 59523; scientific name: Pelea capreolus; NCBI TaxID: 59552; scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationListmonohydroxylated proline; iodoacetamide derivatized residue; deamidated residue
Instrumentultraflex; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-13 03:23:06ID requested
12021-03-23 03:57:08announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: collagen peptide mass fingerprinting, ZooMS, LC-MS/MS, animal husbandry, southern African archaeology
Contact List
Matthew Collins
contact affiliationSection for Evolutionary Genomics, GLOBE Institute, University of Copenhagen, Copenhagen, Denmark; McDonald Institute for Archaeological Research, University of Cambridge, Cambridge, UK
contact emailmatthew.collins@sund.ku.dk
lab head
Meaghan Mackie
contact affiliationUniversity of Copenhagen
contact emailmeaghan@palaeome.org
dataset submitter
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Dataset FTP location
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