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PXD021895-2

PXD021895 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHijacking of transcriptional condensates by endogenous retroviruses
DescriptionHeterochromatin plays essential roles in repressing retrotransposons, e.g. endogenous retroviruses (ERVs) during mammalian development, but the contribution of retrotransposition to lethality observed in embryonic cells deficient for heterochromatin-mediated ERV repression is poorly understood. Here we report that selective degradation of the TRIM28 heterochromatin adapter protein leads to reduced association of transcriptional condensates with loci encoding super-enhancer -driven pluripotency genes in embryonic stem cells, a collapse of the pluripotency transcriptional circuit, and a pre-lethal restriction of pluripotent lineages in mouse embryos. De-repressed ERVs recruit transcriptional condensates in the absence of TRIM28, and ERV RNA facilitates condensation of RNA Polymerase II in vitro. We propose that retrotransposons contribute to the genomic distribution of nuclear condensates, and that RNA species may facilitate “hijacking” of transcriptional condensates in various developmental and disease contexts.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:38.382.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Meierhofer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-08 06:47:43ID requested
12022-07-08 06:38:05announced
22023-11-14 08:51:38announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: transposable elements,heterochromatin, transcriptional condensates
Contact List
Alexander Meissner
contact affiliationMax Planck Institute for Molecular Genetics Department of Genome Regulation Ihnestraße 63-73 14195 Berlin, Germany
contact emailmeissner@molgen.mpg.de
lab head
David Meierhofer
contact affiliationMass Spectrometry Facility MPIMG
contact emailmeierhof@molgen.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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