PXD021895-1

PXD021895 is an original dataset announced via ProteomeXchange.

Title	Hijacking of transcriptional condensates by endogenous retroviruses
Description	Heterochromatin plays essential roles in repressing retrotransposons, e.g. endogenous retroviruses (ERVs) during mammalian development, but the contribution of retrotransposition to lethality observed in embryonic cells deficient for heterochromatin-mediated ERV repression is poorly understood. Here we report that selective degradation of the TRIM28 heterochromatin adapter protein leads to reduced association of transcriptional condensates with loci encoding super-enhancer -driven pluripotency genes in embryonic stem cells, a collapse of the pluripotency transcriptional circuit, and a pre-lethal restriction of pluripotent lineages in mouse embryos. De-repressed ERVs recruit transcriptional condensates in the absence of TRIM28, and ERV RNA facilitates condensation of RNA Polymerase II in vitro. We propose that retrotransposons contribute to the genomic distribution of nuclear condensates, and that RNA species may facilitate “hijacking” of transcriptional condensates in various developmental and disease contexts.
HostingRepository	PRIDE
AnnounceDate	2022-07-08
AnnouncementXML	Submission_2022-07-08_06:38:04.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-08 06:47:43	ID requested
⏵ 1	2022-07-08 06:38:05	announced
2	2023-11-14 08:51:38	announced	2023-11-14: Updated project metadata.

10.1038/S41588-022-01132-W;

submitter keyword: heterochromatin, transposable elements, transcriptional condensates

Alexander Meissner
contact affiliation	Max Planck Institute for Molecular Genetics Department of Genome Regulation Ihnestraße 63-73 14195 Berlin, Germany
contact email	meissner@molgen.mpg.de
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021895
     1. Label: PRIDE project
     2. Name: Hijacking of transcriptional condensates by endogenous retroviruses