PXD021150-2

PXD021150 is an original dataset announced via ProteomeXchange.

Title	MRM quantification of circulating CFP-10 in TB patients
Description	Liquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising method in detecting pathogen derived antigens which are at extremely low concentrations, such as mycobacterium tuberculosis (Mtb) secreted 10 kDa culture filtrate antigen (CFP-10). We employed an optimum denaturing condition for trypsin digestion and imunoprecipitation of targeted peptides and applied this method in clinical samples.
HostingRepository	PanoramaPublic
AnnounceDate	2022-04-14
AnnouncementXML	Submission_2022-04-14_20:13:41.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Qingbo Shu
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	Label:13C(6)15N(4)
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 15:39:14	ID requested
1	2022-02-28 16:31:39	announced
⏵ 2	2022-04-14 20:13:42	announced	: Added publication

Shu Q, Liu S, Alonzi T, LaCourse SM, Singh DK, Bao D, Wamalwa D, Jiang L, Lyon CJ, John-Stewart G, Kaushal D, Goletti D, Hu T, Assay design for unambiguous identification and quantification of circulating pathogen-derived peptide biomarkers. Theranostics, 12(6):2948-2962(2022) [pubmed]

submitter keyword: CFP10 plasma TB

Tony Hu
contact affiliation	Tulane University
contact email	tonyhu@tulane.edu
lab head
Qingbo Shu
contact affiliation	Tulane University
contact email	qshu@tulane.edu
dataset submitter

Panorama Public dataset URI