PXD021150-2
PXD021150 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | MRM quantification of circulating CFP-10 in TB patients |
Description | Liquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising method in detecting pathogen derived antigens which are at extremely low concentrations, such as mycobacterium tuberculosis (Mtb) secreted 10 kDa culture filtrate antigen (CFP-10). We employed an optimum denaturing condition for trypsin digestion and imunoprecipitation of targeted peptides and applied this method in clinical samples. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2022-04-14 |
AnnouncementXML | Submission_2022-04-14_20:13:41.213.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Qingbo Shu |
SpeciesList | scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773; |
ModificationList | Label:13C(6)15N(4) |
Instrument | TSQ Altis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-08-26 15:39:14 | ID requested | |
1 | 2022-02-28 16:31:39 | announced | |
⏵ 2 | 2022-04-14 20:13:42 | announced | : Added publication |
Publication List
Shu Q, Liu S, Alonzi T, LaCourse SM, Singh DK, Bao D, Wamalwa D, Jiang L, Lyon CJ, John-Stewart G, Kaushal D, Goletti D, Hu T, Assay design for unambiguous identification and quantification of circulating pathogen-derived peptide biomarkers. Theranostics, 12(6):2948-2962(2022) [pubmed] |
Keyword List
submitter keyword: CFP10 plasma TB |
Contact List
Tony Hu | |
---|---|
contact affiliation | Tulane University |
contact email | tonyhu@tulane.edu |
lab head | |
Qingbo Shu | |
contact affiliation | Tulane University |
contact email | qshu@tulane.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |