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PXD021150-1

PXD021150 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MRM quantification of circulating CFP-10 in TB patients
Description	Liquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising method in detecting pathogen derived antigens which are at extremely low concentrations, such as mycobacterium tuberculosis (Mtb) secreted 10 kDa culture filtrate antigen (CFP-10). We employed an optimum denaturing condition for trypsin digestion and imunoprecipitation of targeted peptides and applied this method in clinical samples.
HostingRepository	PanoramaPublic
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_16:31:39.295.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Qingbo Shu
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	Label:13C(6)15N(4)
Instrument	TSQ Altis

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 15:39:14	ID requested
⏵ 1	2022-02-28 16:31:39	announced
2	2022-04-14 20:13:42	announced	: Added publication

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: CFP10 plasma TB

Contact List

Tony Hu
contact affiliation	Tulane University
contact email	tonyhu@tulane.edu
lab head
Qingbo Shu
contact affiliation	Tulane University
contact email	qshu@tulane.edu
dataset submitter

Full Dataset Link List

Panorama Public dataset URI