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PXD021150 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMRM quantification of circulating CFP-10 in TB patients
DescriptionLiquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising method in detecting pathogen derived antigens which are at extremely low concentrations, such as mycobacterium tuberculosis (Mtb) secreted 10 kDa culture filtrate antigen (CFP-10). We employed an optimum denaturing condition for trypsin digestion and imunoprecipitation of targeted peptides and applied this method in clinical samples.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterQingbo Shu
SpeciesList scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
InstrumentTSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-26 15:39:14ID requested
12022-02-28 16:31:39announced
22022-04-14 20:13:42announced: Added publication
Publication List
Shu Q, Liu S, Alonzi T, LaCourse SM, Singh DK, Bao D, Wamalwa D, Jiang L, Lyon CJ, John-Stewart G, Kaushal D, Goletti D, Hu T, Assay design for unambiguous identification and quantification of circulating pathogen-derived peptide biomarkers. Theranostics, 12(6):2948-2962(2022) [pubmed]
Keyword List
submitter keyword: CFP10 plasma TB
Contact List
Tony Hu
contact affiliationTulane University
contact emailtonyhu@tulane.edu
lab head
Qingbo Shu
contact affiliationTulane University
contact emailqshu@tulane.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI