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PXD019141-2

PXD019141 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStress-induced translation inhibition through displacement of scanning initiation factors
DescriptionCellular responses to environmental stress are frequently mediated by RNA-binding proteins (RBPs). Here, we examined global RBP dynamics in Saccharomyces cerevisiae in response to glucose starvation and heat shock. Each stress induced rapid remodeling of the protein:RNA interactome, without corresponding changes in RBP abundance. Consistent with general translation shutdown, ribosomal proteins contacting the mRNA showed decreased RNA-association. Among translation components, RNA-association was most reduced for initiation factors involved in 40S scanning (eIF4A, eIF4B, and Ded1), indicating a common mechanism of translational repression. In unstressed cells, eIF4A, eIF4B, and Ded1 primarily targeted the 5′-ends of mRNAs. Following glucose withdrawal, mRNAs remained stable, but 5’-binding was abolished within 30sec, explaining the rapid translation shutdown. Heat shock induced progressive loss of 5’ RNA-binding by initiation factors over ~16min, and translation shutoff provoked 5′-degradation by Xrn1, selectively for mRNAs encoding translation-related factors. These results reveal mechanisms underlying translational control of gene expression during stress.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:13:20.422.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStefan Bresson
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-12 19:03:52ID requested
12020-10-13 05:27:05announced
22024-10-22 05:13:20announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: translational control., heat shock, glucose starvation, environmental stress, initiation factors,RNA-binding proteins, mRNA, translation
Contact List
David Tollervey
contact affiliationUniversity of Edinburgh
contact emaild.tollervey@ed.ac.uk
lab head
Stefan Bresson
contact affiliationUniversity of Edinburgh
contact emailstefan.bresson@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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