PXD019141-1

PXD019141 is an original dataset announced via ProteomeXchange.

Title	Stress-induced translation inhibition through displacement of scanning initiation factors
Description	Cellular responses to environmental stress are frequently mediated by RNA-binding proteins (RBPs). Here, we examined global RBP dynamics in Saccharomyces cerevisiae in response to glucose starvation and heat shock. Each stress induced rapid remodeling of the protein:RNA interactome, without corresponding changes in RBP abundance. Consistent with general translation shutdown, ribosomal proteins contacting the mRNA showed decreased RNA-association. Among translation components, RNA-association was most reduced for initiation factors involved in 40S scanning (eIF4A, eIF4B, and Ded1), indicating a common mechanism of translational repression. In unstressed cells, eIF4A, eIF4B, and Ded1 primarily targeted the 5′-ends of mRNAs. Following glucose withdrawal, mRNAs remained stable, but 5’-binding was abolished within 30sec, explaining the rapid translation shutdown. Heat shock induced progressive loss of 5’ RNA-binding by initiation factors over ~16min, and translation shutoff provoked 5′-degradation by Xrn1, selectively for mRNAs encoding translation-related factors. These results reveal mechanisms underlying translational control of gene expression during stress.
HostingRepository	PRIDE
AnnounceDate	2020-10-13
AnnouncementXML	Submission_2020-10-13_05:27:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Bresson
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 19:03:52	ID requested
⏵ 1	2020-10-13 05:27:05	announced
2	2024-10-22 05:13:20	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: RNA-binding proteins, environmental stress, heat shock, glucose starvation, mRNA, translation, initiation factors, translational control.

David Tollervey
contact affiliation	University of Edinburgh
contact email	d.tollervey@ed.ac.uk
lab head
Stefan Bresson
contact affiliation	University of Edinburgh
contact email	stefan.bresson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD019141

PRIDE project URI

• PRIDE
  1. PXD019141
     1. Label: PRIDE project
     2. Name: Stress-induced translation inhibition through displacement of scanning initiation factors