PXD018580-2

PXD018580 is an original dataset announced via ProteomeXchange.

Title	Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping
Description	Monoamine oxidases MAOA and MAOB catalyze important cellular functions such as the deamination of neurotransmitters. Correspondingly, MAO inhibitors are used for the treatment of severe neuropsychiatric disorders such as depression. A commonly prescribed drug against refractory depression is tranylcypromine, however, the side effects are poorly understood. In order to decipher putative off-targets, we synthesized two tranylcypromine probes equipped with either an alkyne moiety or an alkyne-diazirine minimal photocrosslinker for in situ proteome profiling. Surprisingly, LC-MS/MS analysis revealed low enrichment of MAOA and relatively promiscuous labeling of proteins. Photoprobe labeling paired with fluorescent imaging studies revealed lysosomal trapping which could be largely reverted by the addition of lysosomotropic drugs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:48.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Drechsel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 06:41:36	ID requested
1	2020-10-19 22:19:04	announced
⏵ 2	2024-10-22 05:13:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, ABPP, LC-MS/MS

Stephan Sieber
contact affiliation	Technical University of Munich
contact email	stepha.sieber@tum.de
lab head
Jonas Drechsel
contact affiliation	TU Munich
contact email	jonas.drechsel@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018580
     1. Label: PRIDE project
     2. Name: Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping