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PXD018580-1

PXD018580 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping
DescriptionMonoamine oxidases MAOA and MAOB catalyze important cellular functions such as the deamination of neurotransmitters. Correspondingly, MAO inhibitors are used for the treatment of severe neuropsychiatric disorders such as depression. A commonly prescribed drug against refractory depression is tranylcypromine, however, the side effects are poorly understood. In order to decipher putative off-targets, we synthesized two tranylcypromine probes equipped with either an alkyne moiety or an alkyne-diazirine minimal photocrosslinker for in situ proteome profiling. Surprisingly, LC-MS/MS analysis revealed low enrichment of MAOA and relatively promiscuous labeling of proteins. Photoprobe labeling paired with fluorescent imaging studies revealed lysosomal trapping which could be largely reverted by the addition of lysosomotropic drugs.
HostingRepositoryPRIDE
AnnounceDate2020-10-20
AnnouncementXMLSubmission_2020-10-19_22:19:03.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonas Drechsel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-15 06:41:36ID requested
12020-10-19 22:19:04announced
22024-10-22 05:13:48announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, LC-MS/MS, ABPP
Contact List
Stephan Sieber
contact affiliationTechnical University of Munich
contact emailstepha.sieber@tum.de
lab head
Jonas Drechsel
contact affiliationTU Munich
contact emailjonas.drechsel@tum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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