PXD018580 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping |
Description | Monoamine oxidases MAOA and MAOB catalyze important cellular functions such as the deamination of neurotransmitters. Correspondingly, MAO inhibitors are used for the treatment of severe neuropsychiatric disorders such as depression. A commonly prescribed drug against refractory depression is tranylcypromine, however, the side effects are poorly understood. In order to decipher putative off-targets, we synthesized two tranylcypromine probes equipped with either an alkyne moiety or an alkyne-diazirine minimal photocrosslinker for in situ proteome profiling. Surprisingly, LC-MS/MS analysis revealed low enrichment of MAOA and relatively promiscuous labeling of proteins. Photoprobe labeling paired with fluorescent imaging studies revealed lysosomal trapping which could be largely reverted by the addition of lysosomotropic drugs. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-20 |
AnnouncementXML | Submission_2020-10-19_22:19:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonas Drechsel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-15 06:41:36 | ID requested | |
⏵ 1 | 2020-10-19 22:19:04 | announced | |
2 | 2024-10-22 05:13:48 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, LC-MS/MS, ABPP |
Contact List
Stephan Sieber |
contact affiliation | Technical University of Munich |
contact email | stepha.sieber@tum.de |
lab head | |
Jonas Drechsel |
contact affiliation | TU Munich |
contact email | jonas.drechsel@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018580
- Label: PRIDE project
- Name: Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping