PXD018578-1

PXD018578 is an original dataset announced via ProteomeXchange.

Title	LETR1 (LINC01197) RNA pull-down in human LECs followed by LC-MSMS
Description	Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. There is ample evidence that distinct types of vasculature undergo tight transcriptional control to preserve their structure, identity, and functions. We determined, for the first time, the global lineage-specific lncRNAome of human dermal blood and lymphatic endothelial cells (BECs and LECs), combining RNA-Seq and CAGE-Seq. A subsequent genome-wide antisense oligonucleotide-knockdown profiling of two BEC- and two LEC-specific lncRNAs identified LETR1 as a critical gatekeeper of the global LEC transcriptome. Deep RNA-DNA and RNA-protein interaction studies, and phenotype rescue analyses revealed that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes governing the growth and migratory ability of LECs. Together, our study provides new evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_03:19:37.707.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018578
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luca Ducoli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 04:51:02	ID requested
⏵ 1	2024-10-11 03:19:38	announced

10.6019/PXD018578;

submitter keyword: Human, LESR2, LC-MSMS, LINC01197, lncRNA, LECs

Michael Detmar
contact affiliation	Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology (ETH) Zurich, 8093 Zurich, Switzerland
contact email	michael.detmar@pharma.ethz.ch
lab head
Luca Ducoli
contact affiliation	ETH Zurich
contact email	luca.ducoli@pharma.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018578

PRIDE project URI

• PRIDE
  1. PXD018578
     1. Label: PRIDE project
     2. Name: LETR1 (LINC01197) RNA pull-down in human LECs followed by LC-MSMS