PXD018529 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shedding lights on the alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: an antiproliferative effect was unveiled |
Description | Antiproliferative effect of ang-(1-7) has been further described and detailed, assessing its possibility as a therapeutic cancer control agent. Other endogenous peptides in this system have been studied and characterized, such as alamandine, a heptapeptide very similar to ang-(1-7) in terms of amino acid sequence and physiological effects. In this study, we described for the first time the anti-proliferative effect induced by alamandine treatment in human cancer cells (Mia Paca-2 and A-549) and its signaling in CHO-MrgD by phosphoproteomics. Our results showed that alamandine inhibited cancer cell growth in vitro assays, but did not in non-cancer cells. Our proteomics results revealed that alamandine reprogramed the energetic metabolism in Mia Paca-2, inhibited PI3K/AKT/mTOR pathways and translocated Fox01 to nucleus portion, not only but also is able to decrease cell growth and possibly migration regulating several proteins related to actin cytoskeleton and focal adhesion. Based in our phosphoproteomics results of alamandine signaling in CHO-MrgD, we have unveiled the anti-proliferative mechanism induced by alamandine/MrgD interaction. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:37:31.822.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thiago Verano-Braga |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-14 02:39:01 | ID requested | |
1 | 2022-06-07 13:08:06 | announced | |
⏵ 2 | 2024-10-22 05:37:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
da Silva FA, Rodrigues-Ribeiro L, Melo-Braga MN, Passos-Silva DG, Sampaio WO, Gorshkov V, Kjeldsen F, Verano-Braga T, Santos RAS, Phosphoproteomic studies of alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: An antiproliferative effect is unveiled. Proteomics, 22(17):e2100255(2022) [pubmed] |
10.1002/pmic.202100255; |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: Mia Paca-2, Human, CHO, Alamandine treatment |
Contact List
Thiago Verano Braga |
contact affiliation | Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. |
contact email | tvbraga@icb.ufmg.br |
lab head | |
Thiago Verano-Braga |
contact affiliation | Federal University of Minas Gerais |
contact email | npf.ufmg@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018529
- Label: PRIDE project
- Name: Shedding lights on the alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: an antiproliferative effect was unveiled