PXD018529-1

PXD018529 is an original dataset announced via ProteomeXchange.

Title	Shedding lights on the alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: an antiproliferative effect was unveiled
Description	Antiproliferative effect of ang-(1-7) has been further described and detailed, assessing its possibility as a therapeutic cancer control agent. Other endogenous peptides in this system have been studied and characterized, such as alamandine, a heptapeptide very similar to ang-(1-7) in terms of amino acid sequence and physiological effects. In this study, we described for the first time the anti-proliferative effect induced by alamandine treatment in human cancer cells (Mia Paca-2 and A-549) and its signaling in CHO-MrgD by phosphoproteomics. Our results showed that alamandine inhibited cancer cell growth in vitro assays, but did not in non-cancer cells. Our proteomics results revealed that alamandine reprogramed the energetic metabolism in Mia Paca-2, inhibited PI3K/AKT/mTOR pathways and translocated Fox01 to nucleus portion, not only but also is able to decrease cell growth and possibly migration regulating several proteins related to actin cytoskeleton and focal adhesion. Based in our phosphoproteomics results of alamandine signaling in CHO-MrgD, we have unveiled the anti-proliferative mechanism induced by alamandine/MrgD interaction.
HostingRepository	PRIDE
AnnounceDate	2022-06-07
AnnouncementXML	Submission_2022-06-07_13:08:05.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Verano-Braga
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Cricetulus griseus; NCBI TaxID: 10029;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 02:39:01	ID requested
⏵ 1	2022-06-07 13:08:06	announced
2	2024-10-22 05:37:33	announced	2024-10-22: Updated project metadata.

da Silva FA, Rodrigues-Ribeiro L, Melo-Braga MN, Passos-Silva DG, Sampaio WO, Gorshkov V, Kjeldsen F, Verano-Braga T, Santos RAS, Phosphoproteomic studies of alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: An antiproliferative effect is unveiled. Proteomics, 22(17):e2100255(2022) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Mia Paca-2, Human, CHO, Alamandine treatment

Thiago Verano Braga
contact affiliation	Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
contact email	tvbraga@icb.ufmg.br
lab head
Thiago Verano-Braga
contact affiliation	Federal University of Minas Gerais
contact email	npf.ufmg@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD018529

PRIDE project URI

• PRIDE
  1. PXD018529
     1. Label: PRIDE project
     2. Name: Shedding lights on the alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: an antiproliferative effect was unveiled