PXD018297 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Lon protease is important for growth under stressful conditions and pathogenicity of the phytopathogen, bacterium Dickeya solani. |
Description | The Lon protein is a protease implicated in virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria include important potato pathogens, with the most aggressive species, D. solani. To determine the importance of Lon for pathogenicity and response to stress conditions of bacteria, we constructed a D. solani Δlon strain. The mutant bacteria showed increased sensitivity to certain stress conditions, in particular osmotic and high-temperature stresses. Furthermore, qPCR analysis showed an increased expression of the lon gene in D. solani under these conditions. The deletion of the lon gene resulted in decreased motility, lower activity of secreted pectinolytic enzymes and finally delayed onset of blackleg symptoms in the potato plants. In the Δlon cells, the altered levels of several proteins, including virulence factors and proteins associated with virulence, were detected by means of MS-SWATCH analysis. These included components of the type III secretion system and proteins involved in bacterial motility. Our results indicate that Lon protease is important for D. solani to withstand stressful conditions and effectively invade the potato plant. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-08_23:41:35.906.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paulina Czaplewska |
SpeciesList | scientific name: Dickeya solani; NCBI TaxID: 1089444; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-31 01:22:59 | ID requested | |
⏵ 1 | 2021-09-08 23:41:36 | announced | |
Publication List
Figaj D, Czaplewska P, Przepi, ó, ra T, Ambroziak P, Potrykus M, Skorko-Glonek J, . Int J Mol Sci, 21(10):(2020) [pubmed] |
Keyword List
submitter keyword: Dickeya solani, LC-MS/MS, SWATH |
Contact List
Paulina Czaplewska |
contact affiliation | Intercollegiate FAculty of Biotechnology University of Gdansk and Medical University of Gdansk |
contact email | pczaplewska@gmail.com |
lab head | |
Paulina Czaplewska |
contact affiliation | University of Gdansk |
contact email | pczaplewska@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018297
- Label: PRIDE project
- Name: Lon protease is important for growth under stressful conditions and pathogenicity of the phytopathogen, bacterium Dickeya solani.