PXD017962-2

PXD017962 is an original dataset announced via ProteomeXchange.

Title	The gain-of-function allele bamAE470K bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly
Description	The outer membrane (OM) of Gram-negative bacteria confers innate resistance to toxins and antibiotics. Integral -barrel outer membrane proteins (OMPs) are assembled into the OM by the beta-barrel assembly machine (BAM), which is composed of one OMP, BamA, and four lipoproteins, BamBCDE. BamBCE can be removed individually with only minor effects on barrier function. However, depletion of BamA or BamD, the only BAM components conserved in all diderm bacteria, causes a global defect in OMP assembly and results in cell death. We have identified a gain-of-function mutation, bamAE470K, that bypasses the requirement for BamD. Although bamD::kan bamAE470K cells exhibit growth defects, they assemble OMPs with surprising robustness. Our results demonstrate that the reactions required for beta-barrel folding and membrane integration are catalyzed solely by BamA.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:19.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meera Gupta
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-10 02:36:04	ID requested
1	2020-07-02 23:31:06	announced
⏵ 2	2024-10-22 05:08:19	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: BamD, outer membrane, mutation, lipoproteins

Martin Wuhr
contact affiliation	Princeton University
contact email	wuhr@princeton.edu
lab head
Meera Gupta
contact affiliation	Princeton University
contact email	meerag@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017962
     1. Label: PRIDE project
     2. Name: The gain-of-function allele bamAE470K bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly