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PXD017962-1

PXD017962 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe gain-of-function allele bamAE470K bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly
DescriptionThe outer membrane (OM) of Gram-negative bacteria confers innate resistance to toxins and antibiotics. Integral -barrel outer membrane proteins (OMPs) are assembled into the OM by the beta-barrel assembly machine (BAM), which is composed of one OMP, BamA, and four lipoproteins, BamBCDE. BamBCE can be removed individually with only minor effects on barrier function. However, depletion of BamA or BamD, the only BAM components conserved in all diderm bacteria, causes a global defect in OMP assembly and results in cell death. We have identified a gain-of-function mutation, bamAE470K, that bypasses the requirement for BamD. Although bamD::kan bamAE470K cells exhibit growth defects, they assemble OMPs with surprising robustness. Our results demonstrate that the reactions required for beta-barrel folding and membrane integration are catalyzed solely by BamA.
HostingRepositoryPRIDE
AnnounceDate2020-07-03
AnnouncementXMLSubmission_2020-07-02_23:31:06.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeera Gupta
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-10 02:36:04ID requested
12020-07-02 23:31:06announced
22024-10-22 05:08:19announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: BamD, mutation, outer membrane, lipoproteins
Contact List
Martin Wuhr
contact affiliationPrinceton University
contact emailwuhr@princeton.edu
lab head
Meera Gupta
contact affiliationPrinceton University
contact emailmeerag@princeton.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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