PXD017870-2

PXD017870 is an original dataset announced via ProteomeXchange.

Title	Membrane proteome of L. amazonensis PH8 and LV79 strains
Description	Leishmaniasis is a serious disease caused by several species of protozoa from Leishmania genus. Parasite molecules that contribute to survival in the insect and vertebrate host are named virulence factors. We have recently shown that L. amazonensis PH8 and LV79 strains have different infectivity in mice and that lesion-derived amastigotes from these two strains differ in terms of proteome. In this project, we compared promastigotes of these two strains in terms of infectivity in vitro. Microsomal membrane fractions of PH8 and LV79 promastigotes were compared using a label-free proteomic approach in search of proteins that could be involved in the higher infectivity of PH8. Among the proteins upregulated in LV79 promastigotes, most of them participate in translation, amino acid metabolism and nucleotide metabolism. On the other hand, the majority of the proteins upregulated in PH8 are involved in carbohydrate metabolism, cytoskeleton composition and vesicle and membrane trafficking.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:37.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppe Palmisano
SpeciesList	scientific name: Leishmania amazonensis; NCBI TaxID: 5659;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-04 02:05:26	ID requested
1	2022-08-09 19:47:18	announced
⏵ 2	2024-10-22 05:38:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: virulence, GP63,Leishmania amazonensis strains, membrane proteome

Beatriz S. Stolf
contact affiliation	Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo
contact email	bstolf@usp.br
lab head
Giuseppe Palmisano
contact affiliation	University of Sao Paulo
contact email	palmisano.gp@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017870
     1. Label: PRIDE project
     2. Name: Membrane proteome of L. amazonensis PH8 and LV79 strains