Updated project metadata. Leishmaniasis is a serious disease caused by several species of protozoa from Leishmania genus. Parasite molecules that contribute to survival in the insect and vertebrate host are named virulence factors. We have recently shown that L. amazonensis PH8 and LV79 strains have different infectivity in mice and that lesion-derived amastigotes from these two strains differ in terms of proteome. In this project, we compared promastigotes of these two strains in terms of infectivity in vitro. Microsomal membrane fractions of PH8 and LV79 promastigotes were compared using a label-free proteomic approach in search of proteins that could be involved in the higher infectivity of PH8. Among the proteins upregulated in LV79 promastigotes, most of them participate in translation, amino acid metabolism and nucleotide metabolism. On the other hand, the majority of the proteins upregulated in PH8 are involved in carbohydrate metabolism, cytoskeleton composition and vesicle and membrane trafficking.