PXD016927 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of global protein acetylation in PRRSV infected pulmonary alveolar macrophages |
Description | Porcine reproductive and respiratory syndrome (PRRS), which caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is a serious viral disease affecting global swine industry. At present, PRRSV vaccines fail to prevent this disease. Consequently, new antiviral strategies to compensate for the inefficacy of available vaccines are urgently required. Lysine acetylation is an important post-translational modification (PTM) regulating an array of pathological and physiological conditions. In this study, we profiled the global acetylome using acetylation specific antibody based enrichment and Tandem mass tag (TMT) label LC-MS in PRRSV-infected pulmonary alveolar macrophages (PAMs). As a result, 3731 lysine acetylation sites on 1421 cellular proteins were identified and quantified 6 hours post infection (hpi). Bioinformatics analysis of the differentially acetylated proteins revealed their involvement in various biological processes, including the host immune response and energy metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-11 |
AnnouncementXML | Submission_2020-12-10_23:18:29.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jianyu Fang |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | acetylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-02 05:24:17 | ID requested | |
⏵ 1 | 2020-12-10 23:18:30 | announced | |
Publication List
Fang J, Qiao S, Wang K, Li R, Wang L, Li H, Zhang G, Quantitative Proteomic Analysis of Global Protein Acetylation in PRRSV-Infected Pulmonary Alveolar Macrophages. Proteomics, 21(2):e2000019(2021) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Porcine reproductive and respiratory syndrome |
Tandem mass tag label LC-MS |
post-translational modification |
acetylation. |
Contact List
Gaiping Zhang |
contact affiliation | Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. |
contact email | zhanggaip@126.com |
lab head | |
Jianyu Fang |
contact affiliation | Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences |
contact email | fangjianyu2006@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016927
- Label: PRIDE project
- Name: Quantitative proteomic analysis of global protein acetylation in PRRSV infected pulmonary alveolar macrophages